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Montagem, caracterização e validação de um endoscópio rígido de tempo de vida de fluorescência para imageamento clínico de lesões de pele
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Even so, FLIM ordinarily needs a far more subtle instrumentation than most techniques depending on the continual-state method, what results in a issue for moving this type of process to the clinical environment. We report the assembly, characterization, validation, and scientific software of a multispectral FLIM program that includes a handheld probe made up of a laser scanning rigid endoscope. The assembled system makes use of a 355 nm limited pulsed laser as excitation and it has three spectral channels, targeting the emission of collagen, NADH, and FAD, which are very important endogenous fluorophores. The procedure acquires pictures of 8.sixty five x eight.sixty five mm2 areas in ~ 2.4 s. MATLAB codes ended up penned to process the images utilizing a biexponential design and also a modified phasor approach. In vivo validation measurements of tumors induced in mice had been carried out. The technique was also validated with in vivo imaging of pores and skin of balanced volunteers. The assembled FLIM system was moved to Hospital Amaral Carvalho, in which we done a pilot scientific analyze, in which different types of skin lesions have been imaged in vivo in the scientific location. An important contrast Monografias was accomplished on seborrheic keratosis, Bowen´s condition, and sclerodermiform basal cell carcinoma tumors. These benefits point out the probable of This system for scientific imaging of skin lesions.